TRAF6 Rabbit Polyclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 60 KDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: RNF85; TNF receptor-associated factor 6; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer; RING finger protein 85; MGC:3310; TRAF6 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： TRAF6 | Protein Name： TNF receptor-associated factor 6 | ||
Gene ID： 7189 (Human) | SwissPro： Q9Y4K3 (Human) | |||
Subcellular Location： Cytoplasm. Cytoplasm, cell cortex. Nucleus. Lipid droplet. | ||||
Immunogen： The antiserum was produced against synthesized peptide derived from human TRAF6. AA range: 105-154. | ||||
Specificity: TRAF6 Polyclonal Antibody detects endogenous levels of TRAF6 protein. | ||||
| Product images | |
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Fig: Immunocytochemistry analysis of Hela cells labeling TRAF6 with Rabbit anti-TRAF6 antibody （AWA57647）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-TRAF6 antibody （AWA57647）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig: Fluorescence immunohistochemical analysis of Mouse-ileum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TRAF6 antibody (AWA57647) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA57647) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig: Fluorescence immunohistochemical analysis of Rat-lung tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TRAF6 antibody (AWA57647) at 1/50 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA57647) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Western blot analysis of TRAF6 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA57647, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell Lane 2: NPK-49F cell Lane 3: HELA cell Predicted band size: 63 kDa Observed band size: 63 kDa |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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