Ki67 Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
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- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 359 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: Antigen KI 67; Antigen KI-67; Antigen Ki67; Ki 67; KI67; KIA; MKI67; MIB; MIB 1; Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： MKI67 | Protein Name： Proliferation marker protein Ki-67 | ||
Gene ID： 4288 (Human) | SwissPro： P46013 (Human) | |||
Subcellular Location： Chromosome. Nucleus. Nucleus, nucleolus. | ||||
Immunogen： Synthetic peptide within human Ki67. | ||||
Specificity: Ki67 Monoclonal Antibody detects endogenous levels of Ki67 protein. | ||||
| Product images | |
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Fig: Multiplex immunohistochemistry analysis of Mouse-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). Merged staining of anti-CD3G (AWA12677; green; TSA-520:AWI0688), anti-Ki67 (AWA11025; red; TSA-570: AWI0689), anti-F4/80 (AWA10493; white; TSA-690: AWI0691). Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of anti-CD3G (AWA12677) (1/200 dilution), anti-Ki67 (AWA11025) (1/200 dilution), anti-F4/80 (AWA10493) (1/200 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version. |
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Fig : Western blot analysis of Ki67 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11025, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LN229 cell Lane 2: MCF-7 cell Lane 3: HEPG2 cell Predicted molecular weight:358KD Observed molecular weight:358KD Exposure time：15seconds |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-KI67 antibody (AWA11025) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11025) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Immunocytochemistry analysis of A431 cells labeling Ki67 with Rabbit anti-Ki67 antibody (AWA11025) at 1/50 dilution (Red ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ki67 antibody (AWA11025) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig: Fluorescence immunohistochemical analysis of mouse-cell line derived tumor xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ki67 antibody (AWA11025) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11025) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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