Iba1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA11292
应用:
WB,IHC-P,IF,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    17 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    AIF 1; AIF1; AIF1 protein; IBa1; iba-1; Iba-1;IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:1000-1:4000
    IF 1:50-1:200
    FCM 1:50-1:100



    Immunogen

    Information

    Gene Name:

    AIF1

    Protein Name:

    Allograft inflammatory factor 1


    Gene ID:

    199 (Human)  
    11629 (Mouse)  
    29427 (Rat)

    SwissPro:

    P55008 (Human)  
    O70200 (Mouse)  
    P55009 (Rat)


    Subcellular Location:

    Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Cell projection, phagocytic cup.


    Immunogen:

    Synthetic peptide within human Iba1.


    Specificity:

    Iba1 Monoclonal Antibody detects endogenous levels of Iba1 protein.




    Product images
    Iba1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Rabbit anti-IBA1 antibody (AWA11292) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11292) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Iba1 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of Iba1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11292, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: THP-1 cell
    Lane 2: J774A.1 cell
    Lane 3: Rat spleen

    Predicted molecular weight:17 kDa
    Observed molecular weight:17 kDa
    Exposure time:90s
    Iba1 Recombinant Rabbit Monoclonal Antibody - 3 Fig: Immunocytochemistry analysis of RAW264.7 cells labeling IBA1 with Rabbit anti-IBA1 antibody (AWA11292) at 1/50 dilution(green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-IBA1 antibody (AWA11292) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    Iba1 Recombinant Rabbit Monoclonal Antibody - 4 Fig: Fluorescence immunohistochemical analysis of Mouse-cerebrum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-IBA1 antibody (AWA11292) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11292) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
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