Iba1 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 17 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: AIF 1; AIF1; AIF1 protein; IBa1; iba-1; Iba-1;IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： AIF1 | Protein Name： Allograft inflammatory factor 1 | ||
Gene ID： 199 (Human) | SwissPro： P55008 (Human) | |||
Subcellular Location： Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Cell projection, phagocytic cup. | ||||
Immunogen： Synthetic peptide within human Iba1. | ||||
Specificity: Iba1 Monoclonal Antibody detects endogenous levels of Iba1 protein. | ||||
| Product images | |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-cerebellum tissue with Rabbit anti-IBA1 antibody (AWA11292) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11292) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Western blot analysis of Iba1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11292, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MOLM-13 cell Lane 2: Pig Liver Predicted molecular weight:17 kDa Observed molecular weight:17 kDa Exposure time：3min |
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Fig : Western blot analysis of Iba1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11292, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: J774A.1 cell Lane 3: Rat spleen Predicted molecular weight:17 kDa Observed molecular weight:17 kDa Exposure time：90s |
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Fig: Immunocytochemistry analysis of RAW264.7 cells labeling IBA1 with Rabbit anti-IBA1 antibody (AWA11292) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-IBA1 antibody (AWA11292) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig: Fluorescence immunohistochemical analysis of Mouse-cerebrum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-IBA1 antibody (AWA11292) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11292) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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