p53 Recombinant Rabbit Monoclonal Antibody

货号：

AWA12699

应用：

WB,FCM

反应性：

Human,Mouse,Rat,Rabbit

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Rabbit

Molecular Wt:

Predicted MW: 44 kDa
Observed MW: 53 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; BCC7; LFS1; BMFS5; TRP53

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:2000
FCM 1:l000

Immunogen
Information

Gene Name:

TP53

Protein Name:

Cellular tumor antigen p53

Gene ID:

7157 (Human)
22059 (Mouse)
24842 (Rat)

SwissPro:

P04637 (Human)
P02340 (Mouse)
P10361 (Rat)

Subcellular Location:

Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Immunogen:

Synthetic peptide within N-terminal human p53.

Specificity:

p53 Monoclonal Antibody detects endogenous levels of p53 protein.

Product images
	Fig : Western blot analysis of p53 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12699, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW620 cell Lane 2: SW480 cell Lane 3: HT29 cell Lane 4: A431 cell Lane 5: Hek293 cell Predicted molecular weight:44 kDa Observed molecular weight:53 kDa Exposure time: 7 seconds
	Fig : Western blot analysis of P53 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12699, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rabbit lung Lane 2: SW480 cell Lane 3: SW620 cell Lane 4: HacaT cell Lane 5: 5637 cell Lane 6: HUH-7 cell Predicted molecular weight:44 kDa Observed molecular weight:53 kDa Exposure time：90S