p53 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12699
应用:
WB,IHC-P,IF-C,IF-T,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    44 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; BCC7; LFS1; BMFS5; TRP53


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:2000
    IHC-P 1:100-1:500
    IF-C 1:50-1:200
    IF-T 1:100-1:500
    FCM 1:l000



    Immunogen

    Information

    Gene Name:

    TP53

    Protein Name:

    Cellular tumor antigen p53


    Gene ID:

    7157 (Human)
    22059 (Mouse)
    24842 (Rat)

    SwissPro:

    P04637 (Human)
    P02340 (Mouse)
    P10361 (Rat)


    Subcellular Location:

    Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.


    Immunogen:

    Synthetic peptide within N-terminal human p53.


    Specificity:

    p53 Monoclonal Antibody detects endogenous levels of p53 protein.




    Product images
    p53 Recombinant Rabbit Monoclonal  Antibody - 1 Fig : Western blot analysis of p53 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12699, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SW620 cell
    Lane 2: SW480 cell
    Lane 3: HT29 cell
    Lane 4: A431 cell
    Lane 5: Hek293 cell

    Exposure time: 7 seconds
    Predicted molecular weight:44KD
    Observed molecular weight:53KD
    p53 Recombinant Rabbit Monoclonal  Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-myocardium tissue with Rabbit anti-P53 (AWA12699) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12699) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    p53 Recombinant Rabbit Monoclonal  Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-P53 (AWA12699) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12699) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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