Cdk6 Recombinant Rabbit Monoclonal Antibody

货号：

AWA10011

应用：

WB,IHC-P,IF-C

反应性：

Human,Mouse

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human, Mouse

Molecular Wt:

Predicted MW: 37 kDa
Observed MW: 37 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine protein kinase PLSTIRE; Crk 2; Crk2; p40; PLSTIRE; STQTL11; CDK 6; CDK6; Cdk6

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:2000
IHC-P 1:100-1:500
IF-C 1:50-1:200

Immunogen
Information

Gene Name:

CDK6

Protein Name:

Cyclin-dependent kinase 6

Gene ID:

1021 (Human)
12571 (Mouse)

SwissPro:

Q00534 (Human)
Q64261 (Mouse)

Immunogen
Information

Subcellular Location:

Cytoplasm. Nucleus. Cell projection, ruffle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Immunogen:

Synthetic peptide within human Cdk6. AA range: 277-326.

Specificity:

Cdk6 Monoclonal Antibody detects endogenous levels of Cdk6 protein.

Product images
	Fig : Western blot analysis of Cdk6 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10011, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: HepG2 cell Lane 3: 786-O cell Lane 4: ACHN cell Predicted molecular weight:37 kDa Observed molecular weight:37 kDa

	Fig : Western blot analysis of Cdk6 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10011, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: Hela cell Lane 3: K562 cell Lane 4: AGS cell Lane 5: 5637 cell Predicted molecular weight:37 kDa Observed molecular weight:37 kDa

	Fig: Immunocytochemistry analysis of Hela cells labeling Cdk6 with Rabbit anti-Cdk6 antibody (AWA10011) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Cdk6 antibody (AWA10011) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-large intestine tissue with Rabbit anti-Cdk6 (AWA10011) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10011) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of Jurkat cells labeling Cdk6 with Rabbit anti-Cdk6 antibody (AWA10011) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Cdk6 antibody (AWA10011) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).