GFAP Recombinant Mouse Monoclonal Antibody

货号：

AWA06001

应用：

WB,IHC-P,IF-C,IF-T

反应性：

Human,Mouse,Rat,Monkey

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat,Monkey

Molecular Wt:

Predicted MW: 50 kDa Observed MW: 52 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

1mg/ml

Other Names:

GFAP; Glial fibrillary acidic protein; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; gfapl; Intermediate filament protein

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:50-1:200
IF-C 1:50-1:200
IF-T 1:100-1:500

Immunogen
Information

Gene Name:

GFAP

Protein Name:

Glial fibrillary acidic protein

Gene ID:

2670 (Human)
14580 (Mouse)
24387 (Rat)

SwissPro:

P14136 (Human)
P03995 (Mouse)
P47819 (Rat)

Subcellular Location:

Cytoplasm.

Immunogen:

Synthetic peptide amino acids 411-422 of human GFAP.

Specificity:

GFAP Monoclonal Antibody detects endogenous levels of GFAP protein.

Product images
	Fig: Immunocytochemistry analysis of U-251MG cells labeling GFAP with Mouse anti-GFAP antibody (AWA06001) at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-GFAP antibody (AWA06001) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of GFAP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06001, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: N-2a cell Lane 2: Rat brain Predicted molecular weight:50 kDa Observed molecular weight:52 kDa Exposure time：45s
	Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-GFAPantibody (AWA06001) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, I0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06001) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-hippocampal formation tissue with Rabbit anti-GFAP antibody (AWA06001) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06001) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.