GFAP Recombinant Mouse Monoclonal Antibody

货号:
AWA06001
应用:
WB,IHC-P,IF-C,IF-T
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    50 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1mg/ml


    Other Names:

    GFAP; Glial fibrillary acidic protein; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; gfapl; Intermediate filament protein


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:50-1:200
    IF-C 1:50-1:200
    IF-T 1:100-1:500



    Immunogen

    Information

    Gene Name:

    GFAP

    Protein Name:

    Glial fibrillary acidic protein


    Gene ID:

    2670 (Human)    
    14580 (Mouse)
    24387 (Rat)

    SwissPro:

    P14136 (Human)    
    P03995 (Mouse)    
    P47819 (Rat)


    Subcellular Location:

    Cytoplasm.


    Immunogen:

    Synthetic peptide amino acids 411-422 of human GFAP.


    Specificity:

    GFAP Monoclonal Antibody detects endogenous levels of GFAP protein.




    Product images
    GFAP Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of GFAP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06001, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: N-2a cell
    Lane 2: Rat brain

    Predicted molecular weight:50 kDa
    Observed molecular weight:52 kDa
    Exposure time:45s
    GFAP Recombinant Mouse Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-GFAPantibody (AWA06001) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, I0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06001) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    GFAP Recombinant Mouse Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Rat-hippocampal formation tissue with Rabbit anti-GFAP antibody (AWA06001) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06001) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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