CD86 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12696
应用:
WB,IHC-P,IF-C,IF-T,IP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    38 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD antigen CD86; B7-2; B7.2; LAB72; CD86


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:5000
    IHC-P 1:100-1:500
    IF-C 1:50-1:200
    IF-T 1:100-1:500
    IP 1-2ug/sample



    Immunogen

    Information

    Gene Name:

    CD86

    Protein Name:

    T-lymphocyte activation antigen CD86


    Gene ID:

    942 (Human)  
    12524 (Mouse)

    SwissPro:

    P42081 (Human)    
    P42082 (Mouse)


    Subcellular Location:

    Cell membrane.


    Immunogen:

    Synthetic peptide within human CD86. AA range: 1-50.


    Specificity:

    CD86 Monoclonal Antibody detects endogenous levels of CD86 protein.




    Product images
    CD86 Recombinant Rabbit Monoclonal Antibody - 1 Fig: Immunocytochemistry analysis of EL-4-B5 cells labeling CD86 with Rabbit anti-CD86 antibody (AWA12696) at 1/100 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-CD86 antibody (AWA12696)at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    CD86 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with rabbit anti-CD86 antibody (AWA12696) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12696) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    CD86 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with rabbit anti-CD86 antibody (AWA12696) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12696) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    CD86 Recombinant Rabbit Monoclonal Antibody - 4 Fig : Western blot analysis of CD86 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12696, 1/4000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: THP-1 cell
    Lane 2: Jurkat cell
    Lane 3: RBL-2H3 cell
    Lane 4: Raw264.7 cell

    Exposure time: 7 seconds
    Predicted molecular weight: 38 kDa
    Observed molecular weight: 72 kDa

    Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86
    CD86 Recombinant Rabbit Monoclonal Antibody - 5 Fig: Fluorescence immunohistochemical analysis of Rat-Hippocampus tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CD86 antibody (AWA12696) at 1/200 dilution.
    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12696) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    CD86 Recombinant Rabbit Monoclonal Antibody - 6 Fig: Fluorescence immunohistochemical analysis of Mouse-lung tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CD86 antibody (AWA12696) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12696) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    CD86 Recombinant Rabbit Monoclonal Antibody - 7 Fig: Fluorescence immunohistochemical analysis of Mouse-Thymus tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CD86 antibody (AWA12696) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12696) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
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