Stathmin 1 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Monkey | Molecular Wt: 17 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: C1orf215; Lag; LAP18; LAP 18; Metablastin; Oncoprotein 18; OP18; OP 18; p18; p19; Phosphoprotein p19; Phosphoprotein 19; PP17; PP19; PR22; Prosolin; Protein Pr22; Pr22 protein; SMN; Stathmin; stathmin 1/oncoprotein 18; STMN1; STMN 1; Stathmin1; Stathmin 1 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： STMN1 | Protein Name： Stathmin | ||
Gene ID： 3925 (Human) | SwissPro： P16949 (Human) | |||
Subcellular Location： Cytoplasm, cytoskeleton. | ||||
Immunogen： Synthetic peptide within human Stathmin 1. AA range: 1-100 to the C-terminus. | ||||
Specificity: Stathmin 1 Monoclonal Antibody detects endogenous levels of Stathmin 1 protein. | ||||
| Product images | |
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Fig : Western blot analysis of Stathmin 1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12932, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell Lane 2: Jurkat cell Lane 3: EL-4-B5 cell Predicted molecular weight: 17 kDa Observed molecular weight: 17 kDa Exposure time：3min |
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Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Stathmin 1 antibody (AWA12932) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12932) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-jejunum tissue with Rabbit anti-Stathmin 1 antibody (AWA12932) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12932) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-Stathmin 1 antibody (AWA12932) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12932) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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