Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488

货号:
AWS0005
应用:
IF,FCM
反应性:
Rabbit
来源:
Goat
  • 50μL
  • ¥260
  • 现货
  • 100μL
  • ¥480
  • 现货
  • 500μL
  • ¥2100
  • 现货
  • 产品概述
  • 参考文献
  • Product Details

     

    Host Species:

    Goat

    Reactivity:

    Rabbit

    Concentration:

    1 mg/mL


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Conjugate:

    Alexa Fluor 488  


    Formulation:

    Liquid in PBS containing 50% glycerol, 1% BSA and 0.05% PC300.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,store in dark,1 year



    Applications

     

    IF 1:100-1:1000

    FCM 1:100-1:1000



    Information

     

    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.



    Product images
     Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 1 Fig: Immunocytochemistry analysis of Hela cells labeling Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
     Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 2 Fig: Immunocytochemistry analysis of Hela cells labeling Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
     Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 3 Fig: Fluorescence immunohistochemical analysis of MOUSE-brain cortex tissue (Formalin/PFA-fixed paraffin-embedded sections). Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃.DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
     Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 4 Fig: Fluorescence immunohistochemical analysis of Mouse-hippocampus tissue (Formalin/PFA-fixed paraffin-embedded sections). Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃.DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
     Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 5 Fig: Fluorescence immunohistochemical analysis of Mouse-hippocampus tissue (Formalin/PFA-fixed paraffin-embedded sections). Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃.DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

     1.  Yang, Min et al. “Lysine demethylase KDM3A alleviates hyperoxia-induced bronchopulmonary dysplasia in mice by promoting ETS1 expression.” Experimental cell research vol. 435,2 (2024): 113945. doi:10.1016/j.yexcr.2024.113945PubMed:38286256

     2.  Chen, Pan et al. “ACSL4 promotes ferroptosis and M1 macrophage polarization to regulate the tumorigenesis of nasopharyngeal carcinoma.” International immunopharmacology vol. 122 (2023): 110629. doi:10.1016/j.intimp.2023.110629 PubMed:37451020

     3. Li, Zengshi et al. “WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.” Scientific reports vol. 14,1 559. 4 Jan. 2024, doi:10.1038/s41598-024-51243-z.  PubMed:38177414

     4. Tian, Zhenyang et al. “LncARSR promotes glioma tumor growth by mediating glycolysis through the STAT3/HK2 axis.” Cytokine vol. 180 (2024): 156663. doi:10.1016/j.cyto.2024.156663.  PubMed:38815522

    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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