Ki67 Rabbit Monoclonal Antibody

货号:
AWA11025
应用:
WB,IHC-P,IF,mIHC,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    359 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    Antigen KI 67; Antigen KI-67; Antigen Ki67; Ki 67; KI67; KIA; MKI67; MIB; MIB 1; Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:1000
    IF-C 1:50-1:200
    IF-T 1:100-1:500
    mIHC 1:200-1:2000
    FCM 1:500-1:1000



    Immunogen

    Information

    Gene Name:

    MKI67

    Protein Name:

    Proliferation marker protein Ki-67


    Gene ID:

    4288 (Human)  
    17345 (Mouse)  
    246042 (Rat)

    SwissPro:

    P46013 (Human)  
    E9PVX6 (Mouse)  
    Q5RJM0 (Rat)


    Subcellular Location:

    Chromosome. Nucleus. Nucleus, nucleolus.


    Immunogen:

    Synthetic peptide within human Ki67.


    Specificity:

    Ki67 Monoclonal Antibody detects endogenous levels of Ki67 protein.




    Product images
    Ki67 Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of Ki67 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11025, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: LN229 cell
    Lane 2: MCF-7 cell
    Lane 3: HEPG2 cell


    Predicted molecular weight:358KD
    Observed molecular weight:358KD

    Exposure time:15seconds
    Ki67 Rabbit Monoclonal Antibody - 2 Fig: Immunocytochemistry analysis of A431 cells labeling Ki67 with Rabbit anti-Ki67 antibody (AWA11025) at 1/50 dilution (Red ).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Ki67 antibody (AWA11025) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    Ki67 Rabbit Monoclonal Antibody - 3 Fig: Fluorescence immunohistochemical analysis of mouse-cell line derived tumor xenograft tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-Ki67 antibody (AWA11025) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11025) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    Ki67 Rabbit Monoclonal Antibody - 4 Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-KI67 antibody (AWA11025) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11025) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg