Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488

货号:
AWS0003
应用:
IF,FCM
反应性:
Mouse
来源:
Goat
  • 50μL
  • ¥260
  • 现货
  • 100μL
  • ¥480
  • 现货
  • 500μL
  • ¥2100
  • 现货
  • 产品概述
  • 参考文献
  • Product Details

     

    Host Species:

    Goat

    Reactivity:

    Mouse

    Concentration:

    1 mg/mL


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Conjugate:

    Alexa Fluor 488   


    Formulation:

    Liquid in PBS containing 50% glycerol, 1% BSA and 0.05% PC300.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,store in dark,1 year



    Applications

     

    IF 1:100-1:1000

    FCM 1:100-1:1000



    Information

     

    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.



    Product images
    Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 1 Fig: Immunocytochemistry analysis of Mouse skin fibroblasts labeling Vimentin with mouse anti-Vimentin antibody (AWA00336)at 1/50 dilution (Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Vimentin antibody (AWA00336)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 - 2 Fig: Immunocytochemistry analysis of HeLa cells labeling Citrate synthase with mouse anti-Citrate synthase antibody (AWA00889) at 1/50 dilution(green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with mouse anti-Citrate synthase antibody (AWA00809) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat anti-Mouse IgG (H+L) Secondary Antibody(Alexa Fluor 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

    1. Wu, Zhifeng et al. “Silencing p75NTR regulates osteogenic differentiation and angiogenesis of BMSCs to enhance bone healing in fractured rats.” Journal of orthopaedic surgery and research vol. 19,1 192. 20 Mar. 2024, doi:10.1186/s13018-024-04653-8.  PubMed:38504358

    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg