Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488
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- 50μL
- ¥260
- 现货
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- 100μL
- ¥480
- 现货
-
- 500μL
- ¥2100
- 现货
Product Details
| Host Species: Goat | Reactivity: Mouse | Concentration: 1 mg/mL |
Clonality: Polyclonal | Isotype: IgG | Conjugate: Alexa Fluor 488 | |
Formulation: Liquid in PBS containing 50% glycerol, 1% BSA and 0.05% PC300. | |||
Purification: Affinity-chromatography | |||
Storage: -20°C,store in dark,1 year | |||
Applications
| IF 1:100-1:1000 FCM 1:100-1:1000 | ||
Information
| Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species. | ||
| Product images | |
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Fig: Immunocytochemistry analysis of Mouse skin fibroblasts labeling Vimentin with mouse anti-Vimentin antibody （AWA00336）at 1/50 dilution (Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Vimentin antibody （AWA00336）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig: Immunocytochemistry analysis of HeLa cells labeling Citrate synthase with mouse anti-Citrate synthase antibody (AWA00889) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with mouse anti-Citrate synthase antibody (AWA00809) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat anti-Mouse IgG (H+L) Secondary Antibody(Alexa Fluor 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
1. Wu, Zhifeng et al. “Silencing p75NTR regulates osteogenic differentiation and angiogenesis of BMSCs to enhance bone healing in fractured rats.” Journal of orthopaedic surgery and research vol. 19,1 192. 20 Mar. 2024, doi:10.1186/s13018-024-04653-8. PubMed：38504358
-
-
- 50μL
- ¥260
- 现货
-
- 100μL
- ¥480
- 现货
-
- 500μL
- ¥2100
- 现货
-
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