SQSTM1/p62 Mouse Monoclonal Antibody

货号:
AWA00001
应用:
WB,IHC-P,IF-C,IF-T,ELISA,FCM
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Products Images
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    48 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1mg/ml


    Other Names:

    P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:1000
    IF-C 1:100-1:1000
    IF-T 1:100-1:500
    ELISA 1:10000
    FCM 1:200-1:400



    Immunogen

    Information

    Gene Name:

    SQSTM1

    Protein Name:

    Sequestosome-1


    Gene ID:

    8878 (Human)  
    18412 (Mouse)  
    113894 (Rat)

    SwissPro:

    Q13501 (Human)  
    Q64337 (Mouse)  
    O08623 (Rat)


    Subcellular Location:

    Cytoplasmic vesicle, autophagosome. Preautophagosomal structure. Cytoplasm, cytosol. Nucleus, PML body. Late endosome. Lysosome. Nucleus. Endoplasmic reticulum. Cytoplasm, myofibril, sarcomere.


    Immunogen:

    Recombinant fragment of human SQSTM1/p62. AA range: 232-356.


    Specificity:

    SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein.




    Product images
    SQSTM1/p62 Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of SQSTM1/p62 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody ( AWA00001, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SHZ-88 cell
    Lane 2: RBL-2H3 cell
    Lane 3: NRF-49F cell
    Lane 4: NIH3T3 cell
    Lane 5: HEK293 cell
    Lane 6: HepG2 cell
    Lane 7: HCT116 cell
    SQSTM1/p62 Mouse Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of Mouse-colon tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 4 Fig: Fluorescence immunohistochemical analysis of Mouse-cortex tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 5 Fig: Fluorescence immunohistochemical analysis of Rat-colon tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 6 Fig: Fluorescence immunohistochemical analysis of Mouse-hippocampus tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 7 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 8 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 9 Fig : Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 10 Fig: Fluorescence immunohistochemical analysis of Rat-cortex tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 11 Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 12 Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Mouse anti-SQSTM1/p62 antibody (AWA00001) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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