Bcl-2 Recombinant Rabbit Monoclonal Antibody
货号：
AWA10156
应用：
WB,IHC-P,IF-C,IF-T,FCM
反应性：
Human,Mouse,Rat
来源：
Rabbit
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 26 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: BCL2; Apoptosis regulator Bcl-2; Bcl-2 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： BCL2 | Protein Name： Apoptosis regulator Bcl-2 | ||
Gene ID： 596 (Human) | SwissPro： P10415 (Human) | |||
Subcellular Location： Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. Cytoplasm. | ||||
Immunogen： Recombinant protein within human Bcl-2. AA range:1-230. | ||||
Specificity: Bcl-2 Monoclonal Antibody detects endogenous levels of Bcl-2 protein. | ||||
| Product images | |
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Fig : Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10156, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raw264.7 cell Lane 2: C2C12 cell Lane 3: NIH3T3 cell Lane 4: Jurkat cell Lane 5: THP-1 cell Lane 6: K562 cell Predicted molecular weight: 26 kDa Observed molecular weight: 26 kDa |
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Fig: Immunocytochemistry analysis of Hela cells labeling Bcl-2 with Rabbit anti-Bcl-2 antibody (AWA10156) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Bcl2 antibody (AWA10156) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-Bcl-2 antibody (AWA10156) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody(AWA10156)at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-Bcl-2 antibody (AWA10156) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody(AWA10156)at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-Brain tissue with Rabbit anti-Bcl-2 antibody (AWA10156) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10156) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Fluorescence immunohistochemical analysis of Mouse-spleen tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Bcl-2 antibody (AWA43352) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA43352) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner |
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Fig: Fluorescence immunohistochemical analysis of Mouse-lung tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Bcl-2 antibody (AWA10156) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10156) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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细胞描述： GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆，而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素，也可产生
细胞描述： GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆，而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素，也可产生
