GAPDH Rabbit Polyclonal Antibody_艾碧维生物官网

货号：

AWA81000

应用：

反应性：

Human,Mouse,Rat,Monkey

来源：

Rabbit

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Monkey

Molecular Wt:

Predicted MW: 36 kDa
Observed MW: 36 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

GAPDH; GAPD; CDABP0047; OK/SW-cl.12; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; Glyceraldehyde-3-phosphate dehydrogenase

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:5000-1:20000

Immunogen
Information

Gene Name:

GAPDH

Protein Name:

Glyceraldehyde-3-phosphate dehydrogenase

Gene ID:

2597 (Human)
14433 (Mouse)
24383 (Rat)

SwissPro:

P04406 (Human)
P16858 (Mouse)
P04797 (Rat)

Subcellular Location:

Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton.

Immunogen:

Synthesized peptide derived from human GAPDH. AA range: 185-199.

Specificity:

GAPDH Polyclonal Antibody detects endogenous levels of GAPDH protein.

Product images
	Fig : Western blot analysis of GAPDH on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA81000, 1/5000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen Lane 2: PC12 cell Lane 3: HSC-T6 cell Lane 4: Rat brain Lane 5: COS7 cell Predicted molecular weight:36 kDa Observed molecular weight:36 kDa Exposure time：3min
	Fig : Western blot analysis of GAPDH on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA81000, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain Lane 2: HepG2 cell Lane 3: Raw264.7 cell Predicted molecular weight:36 kDa Observed molecular weight:36 kDa