HMGB1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10032
应用:
WB,IHC-P,IF-C,IF-T,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    25 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    high mobility group box 1; High mobility group protein 1; High mobility group protein B1; HMG 1; HMG1; HMG3; HMGB1; SBP 1

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:50000

    IHC-P 1:200-1:2000

    IF-C 1:50-1:500

    IF-T 1:100

    FCM 1:50-1:1000

     



    Immunogen

    Information

    Gene Name:

    HMGB1

    Protein Name:

    High mobility group protein B1


    Gene ID:

    3146 (Human)     

    15289 (Mouse)     

    25459 (Rat)

     

    SwissPro

    P09429 (Human)     

    P63158 (Mouse)     

    P63159 (Rat)


    Subcellular Location:

    Cytoplasm, Nucleus, Cell membrane, Secreted, Chromosome.


    Immunogen:

    Synthetic peptide within Human HMGB1. AA range: 151-200.


    Specificity:

    HMGB1 Monoclonal Antibody detects endogenous levels of HMGB1 protein.




    Product images
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-HMGB1 (AWA10032) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10032) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-HMGB1 (AWA10032) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10032) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Rat-large intesstine tissue with Rabbit anti-HMGB1 (AWA10032) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10032) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 4 Fig : Immunohistochemical analysis of paraffin-embedded Rat-liver tissue with Rabbit anti-HMGB1 (AWA10032) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10032) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 5 Fig : Western blot analysis of HMGB1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10032, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: NIH/3T3 cell lysate
    Lane 2: A2058 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: HEPA1-6 cell lysate
    Lane 5: U251 cell lysate
    Lane 6: GL261 cell lysate
    Lane 7: K562 cell lysate
    Lane 8: RBL-2H3 cell lysate
    Lane 9: PC12 cell lysate
    Lane 10: Jurkat cell lysate

    Predicted molecular weight: 24.9KD
    Observed molecular weight: 26 KD
    Exposure time: 7 seconds
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 6 Fig : Western blot analysis of HMGB1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10032, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell lysate
    Lane 2: HEK293T cell lysate
    Lane 3: C2C12 cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: A375 cell lysate
    Lane 6: MCF-7 cell lysate
    Lane 7: SHZ-88 cell lysate
    Lane 8: 4T1 cell lysate
    Lane 9: COS-7 cell lysate
    Lane 10: HCT116 cell lysate
    Lane 11: MC38 cell lysate

    Predicted molecular weight: 24.9KD
    Observed molecular weight: 26 KD
    Exposure time: 7 seconds
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 7 Fig: Immunocytochemistry analysis of HELA cells labeling HMGB1 with rabbit anti-HMGB1 antibody (AWA10032) at 1/50 dilution(green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-HMGB1 antibody (AWA10032) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488 AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    HMGB1 Recombinant Rabbit Monoclonal Antibody - 8 Fig: Immunocytochemistry analysis of Raw264.7 cells labeling HMGB1 with rabbit anti-HMGB1 antibody (AWA10032) at 1/50 dilution(green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-HMGB1 antibody (AWA10032) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488 AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
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