α-SMA Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10574

应用：

WB,IHC-P,IF-T,FCM,mIHC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

42 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:50000

IHC-P 1:100-1:500

IF-T 1:100-1:500

FCM 1:1000-1:2000

mIHC 1:2000-1:10000

Immunogen

Information

Gene Name：

ACTA2

Protein Name：

Actin, aortic smooth muscle

Gene ID：

59 (Human)

11475 (Mouse)

81633 (Rat)

SwissPro：

P62736 (Human)

P62737 (Mouse)

P62738 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

Synthetic peptide within N-terminal human α-SMA.

Specificity:

α-SMA Monoclonal Antibody detects endogenous levels of α-SMA protein.

Product images
	Fig : Western blot analysis of α-SMA on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10574, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell Lane 2: A549 cell Lane 3: A431 cell Lane 4: LOVO cell Lane 5: HepG2 cell Lane 6: NIH3T3 cell Lane 7: MC38 cell Lane 8: Rat brain tissue Lane 9: HSC-T6 cell Predicted molecular weight:42KD Observed molecular weight:42KD Exposure time:15 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-α-SMA antibody (AWA10574) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10574) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-α-SMA antibody (AWA10574) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10574) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Fluorescence immunohistochemical analysis of Mouse-lung tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-α-SMA antibody (AWA10574) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10574) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.