COX IV Rabbit Polyclonal Antibody

货号:
AWA61720
应用:
WB,IHC-P,IF,IP,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    20 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    COX4I1; COX4; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:5000
    IHC-P 1:50-1:200
    IF 1:50-1:200
    IP 1-2ug/sample
    ELISA 1:20000



    Immunogen

    Information

    Gene Name:

    COX4I1

    Protein Name:

    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial


    Gene ID:

    1327 (Human)
    12857 (Mouse)
    29445 (Rat)

    SwissPro:

    P13073 (Human)
    P19783 (Mouse)
    P10888 (Rat)


    Subcellular Location:

    Mitochondrion inner membrane.


    Immunogen:

    Recombinant protein within human COX IV. AA range: 23-98.


    Specificity:

    COX IV Polyclonal Antibody detects endogenous levels of COX IV protein.




    Product images
    COX IV Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of COX IV on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA61720, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 5637 cell
    Lane 2: U87-MG cell
    Lane 3: SK-BR-3 cell
    Lane 4: HELA cell
    Lane 5: THP-1 cell
    Lane 6: HEPG2 cell
    Lane 7: A549 cell
    Lane 8: 4T1 cell
    Lane 9: GL261 cell

    Predicted molecular weight:20 kDa
    Observed molecular weight:17 kDa
    Exposure time:45seconds
    COX IV Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Kidney tissue with Rabbit anti-COX Ⅳ antibody (AWA61720) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA61720) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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