SEC61B Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA59106

应用：

WB,IF

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥520 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

10 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Sec61 beta subunit; SEC61B; Protein transport protein Sec61 subunit beta

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IF 1:50-1:200

Immunogen

Information

Gene Name：

SEC61B

Protein Name：

Protein transport protein Sec61 subunit beta

Gene ID：

10952 (Human)

66212 (Mouse)

298068 (Rat)

SwissPro：

P60468 (Human)

Q9CQS8 (Mouse)

F7F271 (Rat)

Subcellular Location：

Endoplasmic reticulum membrane.

Immunogen：

Synthesized peptide derived from human SEC61B. AA range: 1-96.

Specificity:

SEC61B Polyclonal Antibody detects endogenous levels of SEC61B protein.

Product images
	Fig : Western blot analysis of SEC61B on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59106, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: Hela cell Lane 3: HepG2 cell Lane 4: A431 cell Lane 5: HepA1-6 cell Lane 6: HSC-T6 cell Lane 7: Mouse Liver Lane 8: Rat Liver Predicted molecular weight:10KD Observed molecular weight:15KD; Exposure time: 7 seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-SEC61B antibody (AWA59106) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA59106) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.