SPARC Rabbit Polyclonal Antibody

货号:
AWA59040
应用:
WB
反应性:
Human,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Rat

    Molecular Wt:

    35 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    Basement membrane protein 40; BM 40; BM-40; ON; Osteonectin; SPARC; ONT; OI17

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

     



    Immunogen

    Information

    Gene Name:

    SPARC

    Protein Name:

    SPARC


    Gene ID:

    6678 (Human)

    24791 (Rat)

    SwissPro:

    P09486 (Human)

    P16975 (Rat)

     


    Subcellular Location:

    Secreted, extracellular space, extracellular matrix, basement membrane.


    Immunogen:

    Synthetic peptide of human SPARC.


    Specificity:

    SPARC Polyclonal Antibody detects endogenous levels of SPARC protein.


    Product images
    SPARC Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of SPARC on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59040, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 786-O cell
    Lane 2: HEPG2 cell
    Lane 3: SK-N-SH cell
    Lane 4: Rat brain tissue

    Predicted molecular weight:35 kDa
    Observed molecular weight:35 kDa

    Exposure time:15 sec
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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