Parkin Rabbit Polyclonal Antibody

货号:
AWA59037
应用:
WB
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    52 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    PARK 2; PARK2; PRKN; PRKN2; E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; Parkinson juvenile disease protein 2; Parkinson disease protein 2; FRA6E; Park2; Parkin 2; AR JP; LPRS 2; LPRS2; PARK2/Parkin; Parkin; PDJ


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000



    Immunogen

    Information

    Gene Name:

    PRKN

    Protein Name:

    E3 ubiquitin-protein ligase parkin


    Gene ID:

    5071 (Human)  
    50873 (Mouse)  
    56816 (Rat)

    SwissPro:

    O60260 (Human)  
    Q9WVS6 (Mouse)  
    Q9JK66 (Rat)


    Subcellular Location:

    Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Mitochondrion. Mitochondrion outer membrane. Cell projection, neuron projection. Postsynaptic density. Presynapse.


    Immunogen:

    Synthetic peptide of human Parkin.


    Specificity:

    Parkin Polyclonal Antibody detects endogenous levels of Parkin protein.




    Product images
    Parkin Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Parkin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59037, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SH-SY5Y cell
    Lane 2: U251 cell Lane 3: Jurkat cell
    Lane 4: K562 cell
    Lane 5: THP-1cell
    Lane 6: Rat brain tissue
    Lane 7: HT22 cell
    Lane 8: N-2A cell

    Predicted molecular weight:52 kDa
    Observed molecular weight:55 kDa / 36 kDa(Isoform)
    Exposure time:45 sec
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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