Cytokeratin 19 Rabbit Polyclonal Antibody

货号:
AWA59018
应用:
WB
反应性:
Human
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human

    Molecular Wt:

    44 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    KRT19; Keratin; type I cytoskeletal 19; Cytokeratin-19; Cytokeratin 19; CK-19; CK 19; CK19; Keratin-19; K19; K1CS

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

     



    Immunogen

    Information

    Gene Name:

    KRT19

    Protein Name:

    Keratin, type I cytoskeletal 19


    Gene ID:

    3880 (Human)     

    SwissPro:

    P08727 (Human)     


    Subcellular Location:

    apicolateral plasma membrane. cell periphery. Costamere. Cytoskeleton. Cytosol. extracellular exosome. intermediate filament. plasma membrane. sarcolemma. terminal web. Z disc.

     


    Immunogen:

    Synthetic peptide of human Cytokeratin 19.


    Specificity:

    Cytokeratin 19 Polyclonal Antibody detects endogenous levels of Cytokeratin 19 protein.


    Product images
    Cytokeratin 19 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Cytokeratin 19 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59018, 1/1000) was used in TBST at room temperature for 2 hours. Goat AntiRabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 5637 cell
    Lane 2: SK-BR-3 cell
    Lane 3: SW480 cell
    Lane 4: SW620 cell
    Lane 5: AGS cell
    Lane 6: HEPG2 cell
    Lane 7: DU145 cell
    Lane 8: MCF-7 cell

    Predicted molecular weight:44KD
    Observed molecular weight:44KD

    Exposure time:45seconds
    Cytokeratin 19 Rabbit Polyclonal Antibody - 2 Fig : Western blot analysis of Cytokeratin 19 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59018, 1/1000) was used in TBST at room temperature for 2 hours. Goat AntiRabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 4T1 cell
    Lane 2: SHZ-88 cell
    Lane 3: RBL-2H3 cell

    Predicted molecular weight:44KD
    Observed molecular weight:44KD

    Exposure time:45seconds
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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