HMGB1 Rabbit Polyclonal Antibody
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-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 25 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: high mobility group box 1; High mobility group protein 1; High mobility group protein B1; HMG 1; HMG1; HMG3; HMGB1; SBP 1
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: HMGB1 | Protein Name: High mobility group protein B1 | ||
Gene ID: 3146 (Human) 15289 (Mouse) 25459 (Rat)
| SwissPro: P09429 (Human) P63158 (Mouse) P63159 (Rat) | |||
Subcellular Location: Cytoplasm, Nucleus, Cell membrane, Secreted, Chromosome. | ||||
Immunogen: Synthetic peptide of human HMGB1. | ||||
Specificity: HMGB1 Polyclonal Antibody detects endogenous levels of HMGB1 protein. |
Product images | |
Fig : Western blot analysis of HMGB1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59010, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHZ-88 cell Lane 2: HCT116 cell Lane 3: MC-38 cell Predicted band size: 25kDa Predicted band size: 25-30kDa Exposure time:90seconds |
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Fig : Western blot analysis of HMGB1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59010, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH3T3 cell Lane 2: A549 cell Lane 3: HEPG2 cell Lane 4: HEPA1-6 cell Lane 5: GL261 cell Lane 6: K562 cell Lane 7: HEK-293 cell Lane 8: C2C12 cell Lane 9: RAW264.7 cell Lane 10: MCF-7 cell Predicted band size: 25kDa Observed band size:25-30kDa Exposure time:90seconds |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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