ZO-1 Rabbit Polyclonal Antibody

货号:
AWA59001
应用:
WB
反应性:
Human,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Rat

    Molecular Wt:

    195 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    Tight junction protein 1; Tight junction protein ZO 1; TJP1; ZO 1; ZO1; ZO-1; Zona occludens protein 1; Zonula occludens protein 1


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000



    Immunogen

    Information

    Gene Name:

    TJP1

    Protein Name:

    Tight junction protein ZO-1


    Gene ID:

    7082 (Human)  
    292994 (Rat)

    SwissPro:

    Q07157 (Human)      
    A6JBM5 (Rat)


    Subcellular Location:

    Cell membrane. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Cell projection, podosome.


    Immunogen:

    Synthetic peptide of human ZO-1.


    Specificity:

    ZO-1 Polyclonal Antibody detects endogenous levels of ZO-1 protein.




    Product images
    ZO-1 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of ZO-1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA59001, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A549 cell
    Lane 2: K562 cell
    Lane 3: LN229 celll

    Predicted molecular weight:195 kDa
    Observed molecular weight:230 kDa
    Exposure time:45 seconds
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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