Calnexin Rabbit Polyclonal Antibody

货号:
AWA58178
应用:
WB,IHC-P,IF
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    68 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    CANX; CNX; Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; FLJ26570


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:500
    IF 1:50-1:200



    Immunogen

    Information

    Gene Name:

    CANX

    Protein Name:

    Calnexin


    Gene ID:

    821 (Human)    
    12330 (Mouse)    
    29144 (Rat)

    SwissPro:

    P27824 (Human)    
    P35564 (Mouse)    
    P35565 (Rat)


    Subcellular Location:

    Endoplasmic reticulum membrane. Mitochondrion membrane. Melanosome membrane.


    Immunogen:

    Synthetic peptide of human Calnexin.


    Specificity:

    Calnexin Polyclonal Antibody detects endogenous levels of Calnexin protein.




    Product images
    Calnexin Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Calnexin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58178, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: PC12 cell
    Lane 3: NIH3T3 cell

    Exposure time: 15 seconds
    Predicted band size: 68 kDa
    Observed band size: 90 kDa

    Calnexin Rabbit Polyclonal Antibody - 2 Fig : Western blot analysis of Calnexin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58178, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: U251 cell
    Lane 2: GL261 cell
    Lane 3: Hela cell
    Lane 4: CAKi-1 cell
    Lane 5: NRK-49F cell
    Lane 6: MOLM-13 cell

    Predicted molecular weight:68 kDa
    Observed molecular weight:90 kDa

    Exposure time:45 sec
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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