Claudin 18.2 Rabbit Polyclonal Antibody

货号：

AWA58066

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 28 kDa
Observed MW: 28 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

claudin 18; Claudin 18; Claudin-18; CLDN18; SFTA5; SFTPJ

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:500
IF-C 1:50-1:200
IF-T 1:100-1:500
FCM 1:50-1:100

Immunogen
Information

Gene Name:

CLDN18

Protein Name:

Claudin-18

Gene ID:

51208 (Human)
56492 (Mouse)
315953 (Rat)

SwissPro:

P56856/P56856-2 (Human)
P56857 (Mouse)
Q5I0E5 (Rat)

Subcellular Location:

Cell membrane, tight junction. Cell membrane. Lateral cell membrane.

Immunogen:

Synthetic peptide corresponding to N terminal of human Claudin 18.2.

Specificity:

Claudin 18.2 Polyclonal Antibody detects endogenous levels of Claudin 18.2 protein.

Product images
	Fig: Immunocytochemistry analysis of A-431 cells labeling Claudin 18 with Rabbit anti-Claudin 18.2 antibody (AWA58066) at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Claudin 18.2 antibody (AWA58066) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Claudin 18.2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58066, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell Lane 2: C2C12 cell Lane 3: Rat heart Predicted molecular weight:28 kDa Observed molecular weight:28 kDa Exposure time:45sec
	Fig: Fluorescence immunohistochemical analysis of Rat-stomach tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Claudin 18.2 antibody (AWA58066) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58066) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.