Claudin 18 Rabbit Polyclonal Antibody

货号:
AWA58066
应用:
WB,IHC-P,IF,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    28 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    claudin 18; Claudin 18; Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC-P 1:100-1:500

    IF 1:50-1:200

    FCM 1:50-1:100

     



    Immunogen

    Information

    Gene Name:

    CLDN18

    Protein Name:

    Claudin-18


    Gene ID:

    51208 (Human)     

    56492 (Mouse)     

    315953 (Rat)

     

    SwissPro:

    P56856 (Human)     

    P56857 (Mouse)     

    Q5I0E5 (Rat)

     


    Subcellular Location:

    Cell membrane, tight junction. Cell membrane. Lateral cell membrane.


    Immunogen:

    Synthetic peptide corresponding to N terminal of Human CLDN18.


    Specificity:

    Claudin 18 Polyclonal Antibody detects endogenous levels of Claudin 18 protein.




    Product images
    Claudin 18 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Claudin 18 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58066, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431 cell
    Lane 2: C2C12 cell
    Lane 3: Rat heart tissue

    Predicted molecular weight:28KD
    Observed molecular weight:28-30KD

    Exposure time:45sec
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