Claudin 18 Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA58066

应用：

WB,IHC-P,IF,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

28 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

claudin 18; Claudin 18; Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:100-1:500

IF 1:50-1:200

FCM 1:50-1:100

Immunogen

Information

Gene Name：

CLDN18

Protein Name：

Claudin-18

Gene ID：

51208 (Human)

56492 (Mouse)

315953 (Rat)

SwissPro：

P56856 (Human)

P56857 (Mouse)

Q5I0E5 (Rat)

Subcellular Location：

Cell membrane, tight junction. Cell membrane. Lateral cell membrane.

Immunogen：

Synthetic peptide corresponding to N terminal of Human CLDN18.

Specificity:

Claudin 18 Polyclonal Antibody detects endogenous levels of Claudin 18 protein.

Product images
	Fig: Immunocytochemistry analysis of A-431 cells labeling Claudin 18 with Rabbit anti-Claudin 18 antibody (AWA58066) at 1/50 dilution(Red ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Claudin 18 antibody (AWA58066) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of Claudin 18 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA58066, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell Lane 2: C2C12 cell Lane 3: Rat heart tissue Predicted molecular weight:28KD Observed molecular weight:28-30KD Exposure time:45sec
	Fig: Fluorescence immunohistochemical analysis of Rat-stomach tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Claudin antibody (AWA58066) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0691). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA58066) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Purple). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.