Nrf2 Rabbit Polyclonal Antibody

货号:
AWA48931
应用:
WB,IHC,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    75-100 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    NFE2L2; NRF2; Nuclear factor erythroid 2-related factor 2; NF-E2-related factor 2; NFE2-related factor 2; HEBP1; Nuclear factor; erythroid derived 2, like 2

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC 1:100-1:300

    IF 1:200-1:1000

    ELISA 1:40000

     



    Immunogen

    Information

    Gene Name:

    NFE2L2 

    Protein Name:

    Nuclear factor erythroid 2-related factor 2


    Gene ID:

    4780 (Human)     

    18024 (Mouse)  

    83619 (Rat)    

     

    SwissPro:

    Q16236 (Human)     

    Q60795 (Mouse)       

    O54968 (Rat)  


    Subcellular Location:

    Cytoplasm, Nucleus.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human Nrf2. AA range: 556-605.

     


    Specificity:

    Nrf2 Polyclonal Antibody detects endogenous levels of Nrf2 protein.




    Product images
    Nrf2 Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of Nrf2 on different s. Protein lysates were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA48931, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-rabbit IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1:Hela cell
    Lane 2: Rat heart tissue
    Lane 3: Rat brain tissue
    Lane 4: Rat liver tissue
    Lane 5: HUVEC cell
    Nrf2 Rabbit Polyclonal Antibody - 2 Fig: Immunocytochemistry analysis of Hela cells labeling Nrf2 with Rabbit anti-Nrf2 antibody (AWA48931)at 1/200 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Nrf2 antibody (AWA48931)at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    Nrf2 Rabbit Polyclonal Antibody - 3 Fig: Fluorescence immunohistochemical analysis of Mouse-retina tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-Nrf2 antibody (AWA48931) at 1/50 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA48931) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    Nrf2 Rabbit Polyclonal Antibody - 4 Fig : Immunohistochemical analysis of paraffin-embedded moue-spleen tissue with Rabbit anti-Nrf2 (AWA48931) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA48931) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Nrf2 Rabbit Polyclonal Antibody - 5 Fig : Western blot analysis of NRF2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA48931, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HEK293 cell
    Lane 2: HCT116 cell
    Lane 3: HepG2 cell
    Lane 4: PC3 cell
    Lane 5: A431 cell
    Lane 6: U251 cell
    Lane 7: MCF-7 cell

    Predicted molecular weight:68kDa
    Observed molecular weight:68,100kDa

    Exposure time:90 sec
    Nrf2 Rabbit Polyclonal Antibody - 6 Fig : Immunohistochemical analysis of paraffin-embedded rat-kidney tissue with Rabbit anti-Nrf2 (AWA48931) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA48931) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Nrf2 Rabbit Polyclonal Antibody - 7 Fig : Immunohistochemical analysis of paraffin-embedded rat-ovary tissue with Rabbit anti-Nrf2 (AWA48931) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA48931) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Nrf2 Rabbit Polyclonal Antibody - 8 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Nrf2 (AWA48931) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA48931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Nrf2 Rabbit Polyclonal Antibody - 9 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-Nrf2 (AWA48931) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA48931) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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