GFAP Rabbit Polyclonal Antibody

货号:
AWA44808
应用:
WB,IF-C,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    50 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    GFAP; Glial fibrillary acidic protein; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; gfapl; Intermediate filament protein


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IF-C 1:50-1:200
    ELISA 1:5000



    Immunogen

    Information

    Gene Name:

    GFAP

    Protein Name:

    Glial fibrillary acidic protein


    Gene ID:

    2670 (Human)    
    14580 (Mouse)
    24387 (Rat)

    SwissPro:

    P14136 (Human)    
    P03995 (Mouse)    
    P47819 (Rat)


    Subcellular Location:

    Cytoplasm.


    Immunogen:

    The antiserum was produced against synthesized peptide derived from human GFAP. AA range: 11-60.


    Specificity:

    GFAP Polyclonal Antibody detects endogenous levels of GFAP protein.




    Product images
    GFAP Rabbit Polyclonal Antibody - 1 Fig : Western blot analysis of GFAP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA44808, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: U251cell
    Lane 2: N-2a cell
    GFAP Rabbit Polyclonal Antibody - 2 Fig: Immunocytochemistry analysis of U251 cells labeling GFAP with Rabbit anti-GFAP antibody (AWA44808)at 1/50 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-GFAP antibody (AWA44808)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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