GFAP Rabbit Polyclonal Antibody
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- 20μL
- ￥620
- 1-3个工作日
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- 50μL
- ￥1250
- 1-3个工作日
-
- 100μL
- ￥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 50 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: GFAP; GFAP_HUMAN; Glial fibrillary acidic protein; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; gfapl; Intermediate filament protein
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:500-1:2000 IF 1:50-1:300 ELISA 1:5000 | |||
Immunogen Information | Gene Name： GFAP | Protein Name： Glial fibrillary acidic protein | ||
Gene ID： 2670 (Human) 14580 (Mouse) 24387 (Rat)
| SwissPro： P14136 (Human) P03995 (Mouse) P47819 (Rat) | |||
Subcellular Location： Cytoplasm. | ||||
Immunogen： The antiserum was produced against synthesized peptide derived from human GFAP. AA range: 11-60. | ||||
Specificity: GFAP Polyclonal Antibody detects endogenous levels of GFAP protein. | ||||
| Product images | |
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Fig : Western blot analysis of GFAP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA44808, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U251cell Lane 2: N-2a cell |
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Fig: Immunocytochemistry analysis of U251 cells labeling GFAP with Rabbit anti-GFAP antibody （AWA44808）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-GFAP antibody （AWA44808）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
-
-
- 20μL
- ￥620
- 1-3个工作日
-
- 50μL
- ￥1250
- 1-3个工作日
-
- 100μL
- ￥2200
- 1-3个工作日
-
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