ERK1/2(Phospho Thr202/Y204) Rabbit Polyclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA44673

应用：

WB,IHC,IF,ELISA

反应性：

Human,Mouse,Rat,Fish

来源：

Rabbit

规格 价格 库存
20μL ￥620 有库存
50μL ￥1250 有库存
100μL ￥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat,Fish

Molecular Wt:

43 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:100-1:300

IF 1:50-1:200

ELISA 1:20000

Not yet tested in other applications.

Immunogen Information

Gene Name：

MAPK1/MAPK3

Protein Name：

Mitogen-activated protein kinase 3

Gene ID：

5595/5594 (Human)

26417/26413 (Mouse)

50689/116590 (Rat)

SwissPro：

P27361/P28482 (Human)

P63085/Q63844 (Mouse)

P21708/P63086 (Rat)

Immunogen：

Synthesized phospho-peptide around the phosphorylation site of human ERK1/2 (phospho Thr202/Y204)

Specificity:

Phospho-ERK1/2 (T202/Y204) Polyclonal Antibody detects endogenous levels of ERK1/2 protein only when phosphorylated at T202 or Y204.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded rat-colon tissue with Rabbit anti-ERK1-2 antibody ( AWA44673 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA44673 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded rat-liver tissue with Rabbit anti-ERK1-2 antibody ( AWA44673 ) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA44673 ) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA44673, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH3T3 cell Lane 2: HELA cell Lane 3: PC-12 cell Lane 4: NPK-49F cell Lane 5: HEK-293 cell Lane 6: RBL-3H3 cell Lane 7: A549 cell Lane 8: U87-MG cell Lane 9: U251 cell Lane 10: HT-22cell Predicted band size: 43 kDa Observed band size: 43 kDa
	Fig: Immunocytochemistry analysis of Hela cells labeling ERK1 2 (phospho Thr202 Y204) with Rabbit anti-ERK1 2 (phospho Thr202 Y204) antibody （AWA44673）at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-ERK1 2 (phospho Thr202 Y204) antibody （AWA44673）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).