Thrombospondin 1 Rabbit Polyclonal Antibody

货号:
AWA42952
应用:
WB,IHC-P
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL 
  • ¥620
  • 有库存
  • 50μL 
  • ¥1250
  • 有库存
  • 100μL 
  • ¥2200
  • 有库存
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    129 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    THBS; THBS 1; THBS1; thrombospondin 1; Thrombospondin-1; TSP; TSP 1; TSP1; TSP-1

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC-p 1:50-1:300



    Immunogen

    Information

    Gene Name:

    THBS1

    Protein Name:

    Thrombospondin-1


    Gene ID:

    7057 (Human)     

    21825 (Mouse)     

    445442 (Rat)

    SwissPro

    P07996 (Human)     

    P35441 (Mouse)     

    Q71SA3 (Rat)

     


    Subcellular Location:

    Endoplasmic reticulum. Sarcoplasmic reticulum.


    Immunogen

    Synthesized peptide derived from human Thrombospondin 1.


    Specificity:

    Thrombospondin 1 Polyclonal Antibody detects endogenous levels of Thrombospondin 1 protein.

     




    Product images
    Thrombospondin 1 Rabbit Polyclonal   Antibody - 1 Fig : Western blot analysis of THBS1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42952, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: N-2A cell
    Lane 2: Rat brain tissue
    Lane 3: RSC96 cell
    Lane 4: HEK293 cell
    Lane 5: PC12 cell
    Lane 6: NRK-49F cell
    Lane 7: K562 cell
    Lane 8: RAW264.7 cell

    Predicted molecular weight:129KD
    Observed molecular weight:129KD
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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