CD16 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 29 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: Fc gamma RIIIa; Fc gamma RIII alpha; Fc gamma RIII; CD16-II; CD16a antigen; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA; FcGRIIIA; FCGR3A; CD16 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: FCGR3A | Protein Name: Low affinity immunoglobulin gamma Fc region receptor III-A | ||
Gene ID: 2214 (Human) | SwissPro: P08637 (Human) | |||
Subcellular Location: Cell membrane. Secreted. | ||||
Immunogen: Recombinant protein within human CD16. AA range: 151-254. | ||||
Specificity: CD16 Monoclonal Antibody detects endogenous levels of CD16 protein. | ||||
Product images | |
Fig : Western blot analysis of CD16 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12698, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW620 cell Lane 2: Jurkat cell Lane 3: THP-1 cell Lane 4: Raw264.7 cell Exposure time: 15 seconds Predicted band size: 29 kDa Observed band size: 50 kDa |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-liver tissue with Rabbit anti-CD16 antibody (AWA12698) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12698) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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