C3 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12697

应用：

WB,IF,FC

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 有库存
50μL ￥1250 有库存
100μL ￥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

187 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ARMD9;ASP;C3;CO3_HUMAN;Complement C3;Complement factor 3;CPAMD1;HEL S 62p;Complement C3c alpha' chain fragment 2;Complement component 3

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IF 1:50-1:100

FC 1:50-1:100

Immunogen Information

Gene Name：

Protein Name：

Complement C3

Gene ID：

718 (Human)

12266 (Mouse)

24232 (Rat)

SwissPro：

P01024 (Human)

P01027 (Mouse)

P01026 (Rat)

Immunogen：

Synthetic peptide within Human C3 aa 1210-1248 / 1663.

Specificity:

C3 Monoclonal Antibody detects endogenous levels of C3 protein.

Product images
	Fig: Immunocytochemistry analysis of HELA cells labeling C3 with Rabbit anti-C3 antibody (AWA12697) at 1/50 dilution(Red ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-C3 antibody (AWA12697) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of C3 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12697, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: Mouse liver Lane 3: Rat liver Predicted molecular weight:187 kDa Observed molecular weight:130/60/37 kDa Exposure time：45s