PD-L1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12695
应用:
WB
反应性:
Human,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Rat

    Molecular Wt:

    33 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    B7 H; B7 H1; B7 homolog 1; B7H1; CD274; CD274 molecule; PD L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PD-L1/CD274; Programmed death ligand 1


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:2000



    Immunogen

    Information

    Gene Name:

    CD274

    Protein Name:

    Programmed cell death 1 ligand 1


    Gene ID:

    29126 (Human)    
    499342 (Rat)

    SwissPro:

    Q9NZQ7 (Human)    
    A6I0W4 (Rat)


    Subcellular Location:

    Cell membrane. Early endosome membrane. Recycling endosome membrane. Nucleus. Endomembrane system. Secreted.


    Immunogen:

    Synthetic peptide within human PD-L1. AA range: 260-290.


    Specificity:

    PD-L1 Monoclonal Antibody detects endogenous levels of PD-L1 protein.




    Product images
    PD-L1 Recombinant Rabbit Monoclonal  Antibody - 1 Fig : Western blot analysis of PD-L1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12695, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: PC3 cell
    Lane 2: A375 cell
    Lane 3: A549 cell
    Lane 4: U87-MG cell
    Lane 5: Hela cell


    Exposure time: 7 min
    Predicted band size: 33 kDa
    Observed band size: 45-50 kDa
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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