HNF-4-alpha Recombinant Rabbit Monoclonal Antibody

货号:
AWA12691
应用:
WB,IHC-P ,IF,FC 
反应性:
Human,Mouse
来源:
Rabbit
  • 20μL 
  • ¥620
  • 有库存
  • 50μL 
  • ¥1250
  • 有库存
  • 100μL
  • ¥2200
  • 有库存
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse

    Molecular Wt:

    53 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:1000

    IHC-P 1:100-1:500

    IF 1:100

    FC 1:50-1:100

     



    Immunogen Information

    Gene Name:

    HNF4A

    Protein Name:

    Hepatocyte nuclear factor 4-alpha


    Gene ID:

    3172 (Human)  

    15378 (Mouse) 

    25735 (Rat)

    SwissPro:

    P41235 (Human)    

    P49698 (Mouse) 

    P22449 (Rat)



    Immunogen:

    Synthetic peptide within Human HNF-4-alpha aa 21-70 / 474.


    Specificity:

    HNF-4-alpha Monoclonal Antibody detects endogenous levels of HNF-4-alpha protein.




    Product images
    HNF-4-alpha Recombinant Rabbit  Monoclonal Antibody - 1 Fig : Western blot analysis of HNF-4-alpha on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA12691, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell lysate
    Lane 2: AML12 cell lysate

    Predicted molecular weight: 52.8KD
    Observed molecular weight: 53 KD
    Exposure time: 7 seconds
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg