pro Caspase-7 Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 有库存
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- 50μL
- ¥1250
- 有库存
-
- 100μL
- ¥2200
- 有库存
Product Details
| Host Species: Rabbit | Reactivity: Human | Molecular Wt: 34 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: CASP7; MCH3; Caspase-7; CASP-7; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3; proCaspase-7; proCaspase7
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000-1:2000 IHC-P 1:50-1:200 IF 1:50-1:200
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Immunogen Information | Gene Name: CASP7 | Protein Name: Caspase7 | ||
Gene ID: 840 (Human) 12369 (Mouse)
| SwissPro: P55210 (Human) P97864 (Mouse)
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Immunogen: Synthetic peptide within Human Caspase 7 AA range: 1-50. | ||||
Specificity: pro Caspase-7 Monoclonal Antibody detects endogenous levels of pro Caspase-7 protein.
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Product images | |
Fig : Western blot analysis of pro caspase 7 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12689, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 4T1 cell Lane 2: SHZ-88 cell Lane 3: HEPA1-6 cell Lane 4: HELA cell Lane 5: HEK-293 cell Lane 6: HEPG2 cell Lane 7: NIH3T3 cell |
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Fig: Immunocytochemistry analysis of HeLa cells labeling pro-caspase7 with rabbit anti-pro-caspase7 antibody ( AWA12689) at 1/50 dilution(red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-pro-caspase7 antibody ( AWA12689) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291). |
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Fig : Immunohistochemical analysis of paraffin-embedded mouse-kidney tissue with rabbit anti-pro-caspase7 antibody ( AWA12689 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12689) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded rat-kidney tissue with rabbit anti-pro-caspase7 antibody ( AWA12689 ) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA12689) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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