CD31 Recombinant Rabbit Monoclonal Antibody

货号:
AWA11937
应用:
WB,IHC-P,IF-C,IF-T,FCM,IP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    83 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:2000
    IHC-P 1:50-1:1000
    IF-C 1:50-1:200
    IF-T 1:50-1:200
    FCM 1:50-1:100
    IP 1-2μg:sample



    Immunogen

    Information

    Gene Name:

    PECAM1

    Protein Name:

    Platelet endothelial cell adhesion molecule


    Gene ID:

    5175 (Human)  
    18613 (Mouse)
    29583 (Rat)

    SwissPro:

    P16284 (Human)  
    Q08481 (Mouse)  
    Q3SWT0 (Rat)


    Subcellular Location:

    Cell membrane. Membrane raft. Cell junction.


    Immunogen:

    Synthetic peptide within human CD31. AA range: 689-738.


    Specificity:

    CD31 Monoclonal Antibody detects endogenous levels of CD31 protein.




    Product images
    CD31 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of CD31 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11937, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1:Jurkat cell
    Lane 2: HEK-293 cell
    Lane 3:NIH3T3 cell
    Lane 4: RBL-2H3 cell
    Predicted band size: 83 kDa
    Observed band size: 110 kDa
    CD31 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of CD31 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA11937, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: RBL-2H3 cell
    Lane 2: Jurkat cell
    Lane 3: NIH3T3 cell

    Exposure time: 90 seconds
    Predicted molecular weight:83 kDa
    Observed molecular weight:110 kDa
    CD31 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue with Rabbit anti-CD31 antibody (AWA11937) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA11937) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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