SP1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10990
应用:
WB,IHC-P,IF-C,IF-T,FCM,ChIP
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    81 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    SP1; SP 1; SP1_HUMAN; Sp1 transcription factor; Transcription factor Sp1; Specificity protein 1; TSFP1; TSFP 1

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:2000

    IHC-P 1:200-1:1000

    IF-C 1:100-1:500

    IF-T 1:100-1:500

    FCM 1:500-1:1000

    ChIP Use 0.5~2 μg for 25 μg of chromatin.

     



    Immunogen

    Information

    Gene Name:

    SP1

    Protein Name:

    Transcription factor Sp1


    Gene ID:

    6667 (Human)     

    20683 (Mouse)     

    24790 (Rat)

     

    SwissPro:

    P08047 (Human)     

    O89090 (Mouse)     

    Q01714 (Rat)

     


    Subcellular Location:

    Nucleus. Cytoplasm.


    Immunogen:

    Synthetic peptide within Human SP1. AA range: 544-588.


    Specificity:

    SP1 Monoclonal Antibody detects endogenous levels of SP1 protein.




    Product images
    SP1 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of SP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10990, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: A431 cell
    Lane 2: HeLa cell
    Lane 3: HEK293 cell
    Lane 4: K562 cell
    Lane 5: MCF-7 cell
    Lane 6: HaCaT cell

    Predicted molecular weight:81KD
    Observed molecular weight:95-105KD

    Exposure time: 7 seconds
    SP1 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue with Rabbit anti-SP1 antibody (AWA10990) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SP1 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-SP1 antibody (AWA10990) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10990) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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