Granulin Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10520

应用：

WB,IHC-P,IF,FC

反应性：

Human,Mouse

来源：

Rabbit

规格 价格 库存
20μL ￥620 有库存
50μL ￥1250 有库存
100μL ￥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse

Molecular Wt:

64 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

Acrogranin; GEP; GP88; granulin; Granulin A Granulin 5; Granulin B Granulin 4; Granulin C Granulin 6; Granulin D Granulin 7; Granulin E; Granulin F Granulin 3; Granulin G Granulin 2; Granulins; GRN; PCDGF; PEPI; PGRN; Proepithelin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:1000

IF 1:100

FC 1:500-1:1000

Immunogen Information

Gene Name：

GRN

Protein Name：

Acrogranin

Gene ID：

2896 (Human)

14824 (Mouse)

29143 (Rat)

SwissPro：

P28799 (Human)

P28798 (Mouse)

P23785 (Rat)

Immunogen：

Recombinant protein.

Specificity:

Granulin Monoclonal Antibody detects endogenous levels of Granulin protein.

Product images
	Fig : Western blot analysis of Granulin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10520, 1/3000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293 cell lysate Lane 2: A2058 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF-7 cell lysate Lane 5: PC-3 cell lysate Lane 6: RM-1 cell lysate Predicted molecular weight: 63.5KD Observed molecular weight: 70KD Exposure time: 45 seconds
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-testis tissue with Rabbit anti-Granulin (AWA10520) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10520) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-Granulin (AWA10520) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10520) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-Granulin (AWA10520) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10520) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.