NCAPD2 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA10323

应用：

WB,IHC,IF

反应性：

Human

来源：

Rabbit

规格 价格 库存
20μL ￥620 有库存
50μL ￥1250 有库存
100μL ￥2200 有库存

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human

Molecular Wt:

157 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CAP-D2;CAPD2;CEP2;CEP250;CNAP1;CND1;Condensin;NCAPD2;Condensin complex subunit 1;eg7;hCAP-D2;hCAPD2;KIAA0159;mKIAA0159

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:50-1:200

IF 1:100-1:200

Immunogen Information

Gene Name：

NCAPD2 CAPD2 CNAP1 KIAA0159

Protein Name：

Condensin complex subunit 1 (Chromosome condensation-related SMC-associated protein 1) (Chromosome-associated protein D2) (hCAP-D2) (Non-SMC condensin I complex subunit D2) (XCAP-D2 homolog)

Gene ID：

SwissPro：

Immunogen：

Recombinant protein within human NCAPD2 aa 51-200/1401.

Specificity:

NCAPD2 Monoclonal Antibody detects endogenous levels of NCAPD2 protein.

Product images
	Fig : Western blot analysis of NCAPD2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10323, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HEK293T cell lysate Lane 2: Hela cell lysate Lane 3: K562 cell lysate Lane 4: Jurkat cell lysate Lane 5: THP-1 cell lysate Lane 6: Raji cell lysate Predicted molecular weight: 157KD Observed molecular weight: 157KD
	Fig: Immunocytochemistry analysis of Hela cells labeling NCAPD2 with Rabbit anti-NCAPD2 antibody （AWA10323）at 1/100 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-NCAPD2 antibody （AWA10323）at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).