CHOP Recombinant Rabbit Monoclonal Antibody
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 19 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: DDIT3; CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; Growth arrest and DNA damage-inducible protein GADD153 | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:1000-1:5000 | |||
Immunogen Information | Gene Name: DDIT3 | Protein Name: DNA damage-inducible transcript 3 protein | ||
Gene ID: 1649 (Human) | SwissPro: P35638 (Human) | |||
Subcellular Location: Cytoplasm. Nucleus. | ||||
Immunogen: Synthetic peptide within human CHOP. AA range: 135-169. | ||||
Specificity: CHOP Monoclonal Antibody detects endogenous levels of CHOP protein. | ||||
Product images | |
Fig : Western blot analysis of CHOP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10322, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell Lane 2: K562 cell Lane 3: HELA cell Lane 4: HEPG2 cell Lane 5: NIH3T3 cell Lane 6: C2C12 cell | |
Fig: Fluorescence immunohistochemical analysis of Mouse-hippocampus tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CHOP antibody (AWA10322) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/100 dilution for 1 hour at room temperature. Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃.DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellar cortex tissue with rabbit anti-CHOP antibody (AWA10322) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-CHOP antibody (AWA10322) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig: Fluorescence immunohistochemical analysis of Mouse-preoptic area tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CHOP antibody (AWA10322) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/100 dilution for 1 hour at room temperature. Goat Anti-RABBIT IgG H&L (iFluor™ 594, AWS0006) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃.DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Rat-hippocampus tissue with rabbit anti-CHOP antibody (AWA10322) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig: Fluorescence immunohistochemical analysis of rat-brain cortex tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-CHOP antibody (AWA10322) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/100 dilution for 1 hour at room temperature. Goat Anti-RABBIT IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃.DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Rat-eyeball tissue with Rabbit anti-CHOP antibody (AWA10322) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Rat-forehead tissue with Rabbit anti-CHOP antibody (AWA10322) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-CHOP antibody (AWA10322) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10322) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
相关产品
-
Cdk6 Recombinant Rabbit Monoclonal Antibody
GAPDH Rabbit Polyclonal Antibody
GFAP Recombinant Mouse Monoclonal Antibody
Ki67 Rabbit Monoclonal Antibody
HMGB1 Recombinant Rabbit Monoclonal Antibody
SQSTM1/p62 Mouse Monoclonal Antibody
Bcl-2 Recombinant Rabbit Monoclonal Antibody
SOD2 Rabbit Polyclonal Antibody