Aggrecan Recombinant Rabbit Monoclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
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- 50μL
- ¥1250
- 1-3个工作日
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- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 261 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: ACAN; Cartilage-specific proteoglycan core protein; Aggrecan core protein 2; Aggrecan 1; AGCAN; AGC1; SEDK; CSPG1; MSK16; CSPGCP; SSOAOD; Aggrecan | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name： ACAN | Protein Name： Aggrecan core protein | ||
Gene ID： 176 (Human) | SwissPro： P16112 (Human) | |||
Subcellular Location： Secreted, extracellular space, extracellular matrix. | ||||
Immunogen： Recombinant protein within human Aggrecan. AA range: 742-881. | ||||
Specificity: Aggrecan Monoclonal Antibody detects endogenous levels of Aggrecan protein. | ||||
| Product images | |
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Fig : Western blot analysis of Aggrecan on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10160, 1/1000) was used in TBST(0.1%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U2OS cell Lane 2: SAOS-2 cell Predicted molecular weight:261 kDa Observed molecular weight:280~300 kDa Exposure time: 45s |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-Aggrecan antibody (AWA10160) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10160) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue with Rabbit anti-Aggrecan antibody (AWA10160) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10160) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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