GFAP Recombinant Rabbit Monoclonal Antibody

一键复制产品信息

货号:
AWA10155
应用:
WB,IHC-P,IHC-F,IF-T,IP,mIHC
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • 参考文献
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    Predicted MW: 50 kDa Observed MW: 50 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml

     

    Other Names:

    GFAP; Glial fibrillary acidic protein; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; gfapl; Intermediate filament protein

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:1000
    IHC-F 1:100-1:200
    IF-T 1:100-1:500
    IP 1-2ug/sample
    mIHC 1:100-1:1000

    Immunogen
    Information

    Gene Name:

    GFAP

    Protein Name:

    Glial fibrillary acidic protein

     

    Gene ID:

    2670 (Human)    
    14580 (Mouse)
    24387 (Rat)

    SwissPro:

    P14136 (Human)    
    P03995 (Mouse)    
    P47819 (Rat)

     

    Subcellular Location:

    Cytoplasm.

     

    Immunogen:

    Synthetic peptide within human GFAP. AA range: 1-50.

     

    Specificity:

    GFAP Monoclonal Antibody detects endogenous levels of GFAP protein.


    Product images
    GFAP Recombinant Rabbit Monoclonal Antibody - 1 Fig: Multiplex immunohistochemistry analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections).

    Merged staining of anti-GFAP (AWA10155; green; TSA-520:AWI0688), anti-MAP2 (AWA58085; red; TSA-570: AWI0689), anti-IBA1 (AWA11292; white; TSA-690: AWI0691).

    Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of GFAP (AWA10155) (1/800 dilution), anti-MAP2 (AWA58085) (1/800 dilution), anti-IBA1 (AWA11292) (1/800 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain.
    Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version.
    GFAP Recombinant Rabbit Monoclonal Antibody - 2 Fig: Multiplex immunohistochemistry analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections).

    Merged staining of anti-GFAP (AWA10155; green; TSA-520:AWI0688), anti-Neun (AWA10318; red; TSA-570: AWI0689), anti-β III tubulin (AWA80027; white; TSA-690: AWI0691).

    Antibody Wash Solution (for mIHC) (AWI0707, 37℃, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. The section was incubated in three rounds of staining; in the order of GFAP (AWA10155) (1/800 dilution), anti-Neun (AWA10318) (1/800 dilution), anti-β III tubulin (AWA80027) (1/800 dilution); each using a separate fluorescent tyramide signal amplification system. DAPI (blue, AWC0291) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual TSA dyes was performed using a pannoramic midi. This image was generated from the hybridoma version.
    GFAP Recombinant Rabbit Monoclonal Antibody - 3 Fig: Fluorescence immunohistochemical analysis of Rat-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GFAP antibody (AWA10155) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    GFAP Recombinant Rabbit Monoclonal Antibody - 4 Fig : Western blot analysis of GFAP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10155, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat Brain

    Exposure time: 7 seconds
    Predicted molecular weight:50 kDa
    Observed molecular weight:50 kDa
    GFAP Recombinant Rabbit Monoclonal Antibody - 5 Fig : Immunohistochemical analysis of paraffin-embedded Rat-urinary bladder tissue with Rabbit anti-GFAP antibody (AWA10155) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    GFAP Recombinant Rabbit Monoclonal Antibody - 6 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Brain tissue with Rabbit anti-GFAP antibody (AWA10155) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    GFAP Recombinant Rabbit Monoclonal Antibody - 7 Fig : Immunohistochemical analysis of paraffin-embedded Rat-Brain tissue with Rabbit anti-GFAP antibody (AWA10155) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    GFAP Recombinant Rabbit Monoclonal Antibody - 8 Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GFAP antibody (AWA10155) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    GFAP Recombinant Rabbit Monoclonal Antibody - 9 Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GFAP antibody (AWA10155) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

    1. Fan, Shuangshi et al. “Molecular prognostic of nine parthanatos death-related genes in glioma, particularly in COL8A1 identification.” Journal of neurochemistry vol. 168,3 (2024): 205-223. doi:10.1111/jnc.16049.

    PubMed:38225203

    2. Li, Wei et al. “4,5-Dimethoxycanthin-6-one Inhibits Glioblastoma Stem Cell and Tumor Growth by Inhibiting TSPAN1 Interaction with TM4SF1.” Neurochemical research vol. 49,10 (2024): 2897-2909. doi:10.1007/s11064-024-04211-y.  PubMed:39060768

    3.  Zhang, Hui et al. “Liu-Shen-Wan inhibits PI3K/Akt and TRPV1 signaling alleviating bone cancer pain in rats.” Cancer biology & therapy vol. 25,1 (2024): 2432098. doi:10.1080/15384047.2024.2432098.  PubMed:39587385

    复制成功!

    内容已复制到剪贴板

    在线客服
    关闭
    小薇 薇薇 小艾 小奥
    联系销售
    技术支持

    image.png

    月琴艾碧维二维码.jpg