GFAP Recombinant Rabbit Monoclonal Antibody
货号：
AWA10155
应用：
WB,IHC-P,IHC-F,mIHC,IF-T,IP
反应性：
Human,Mouse,Rat
来源：
Rabbit
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- 20μL
- ￥620
- 1-3个工作日
-
- 50μL
- ￥1250
- 1-3个工作日
-
- 100μL
- ￥2200
- 1-3个工作日
Product Details
| Host Species: Rabbit | Reactivity: Human,Mouse,Rat | Molecular Wt: 50 kDa | |
Clonality: Monoclonal | Isotype: IgG | Concentration: 1 mg/ml | ||
Other Names: GFAP; GFAP_HUMAN; Glial fibrillary acidic protein; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; gfapl; Intermediate filament protein
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Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: -20°C,1 year | ||||
Applications
| WB 1:1000-1:5000 IHC-P 1:50-1:1000 IHC-F 1:100-1:200 mIHC 1:1000-1:10000 IF-T 1:50-1:200 IP Use at an assay dependent concentration.
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Immunogen Information | Gene Name： GFAP | Protein Name： Glial fibrillary acidic protein | ||
Gene ID： 2670 (Human) 14580 (Mouse) 24387 (Rat)
| SwissPro： P14136 (Human) P03995 (Mouse) P47819 (Rat) | |||
Subcellular Location： Cytoplasm. | ||||
Immunogen： Synthetic peptide within Human GFAP. AA range: 1-50. | ||||
Specificity: GFAP Monoclonal Antibody detects endogenous levels of GFAP protein. | ||||
| Product images | |
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Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GFAP antibody ( AWA10155 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-urinary bladder tissue with Rabbit anti-GFAP antibody (AWA10155) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GFAP antibody ( AWA10155 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig: Fluorescence immunohistochemical analysis of Rat-cerebellum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-GFAP antibody ( AWA10155 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner. |
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Fig : Western blot analysis of GFAP on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10155, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat Brain Exposure time: 7 seconds Predicted molecular weight:50KD Observed molecular weight:50KD |
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Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Brain tissue with Rabbit anti-GFAP antibody (AWA10155) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-Brain tissue with Rabbit anti-GFAP antibody (AWA10155) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10155) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
1.Fan, Shuangshi et al. “Molecular prognostic of nine parthanatos death-related genes in glioma, particularly in COL8A1 identification.” Journal of neurochemistry vol. 168,3 (2024): 205-223. doi:10.1111/jnc.16049.
-
-
- 20μL
- ￥620
- 1-3个工作日
-
- 50μL
- ￥1250
- 1-3个工作日
-
- 100μL
- ￥2200
- 1-3个工作日
-
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细胞描述： 胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。当细胞从低密度生长到满瓶时，S-100产量增加10倍。 细胞特性： 1）来源：大鼠，胶质瘤 2）形态：成纤维细胞样，贴壁生长 3）规格：1×10 6 cells
细胞描述： GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆，而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素，也可产生
细胞描述： 胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。当细胞从低密度生长到满瓶时，S-100产量增加10倍。 细胞特性： 1）来源：大鼠，胶质瘤 2）形态：成纤维细胞样，贴壁生长 3）规格：1×10 6 cells
