TBR1 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10109
应用:
WB,IHC-P,IHC-F,IF-T,mIHC,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    74 kDa


    Clonality:

    Monoclonal 

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    T box brain protein 1; T-box brain protein 1; T box brain 1; T brain 1; T-brain-1; TBR 1, TBR1, TBR-1; Tbr1; TBR1_HUMAN; TES 56; TES-56

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000

    IHC-P 1:200-1:1000

    IHC-F 1:200-1:1000

    IF-T 1:500

    mIHC 1:1000

    FCM 1:50-1:100

     



    Immunogen

    Information

    Gene Name:

    TBR1

    Protein Name:

    T-box brain protein 1


    Gene ID:

    10716 (Human)     

    21375 (Mouse)     

    680427 (Rat)

     

    SwissPro:

    Q16650 (Human)     

    Q64336 (Mouse)     

    D4A6N8 (Rat)

     


    Subcellular Location:

    Nucleus.


    Immunogen:

    Synthetic peptide within Human TBR1. AA range: 30-75.


    Specificity:

    TBR1 Monoclonal Antibody detects endogenous levels of TBR1 protein.




    Product images
    TBR1 Recombinant Rabbit Monoclonal Antibody - 1 Fig: Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-TBR1 (AWA10109) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    TBR1 Recombinant Rabbit Monoclonal Antibody - 2 Fig: Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-TBR1 (AWA10109) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    TBR1 Recombinant Rabbit Monoclonal Antibody - 3 Fig: Western blot analysis of TBR1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10109, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat brain tissue lysate

    Predicted molecular weight: 74KD
    Observed molecular weight: 74KD
    Exposure time: 90 second
    TBR1 Recombinant Rabbit Monoclonal Antibody - 4 Fig: Western blot analysis of TBR1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10109, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat brain tissue lysate

    Predicted molecular weight: 74KD
    Observed molecular weight: 74KD
    Exposure time: 90 second
    TBR1 Recombinant Rabbit Monoclonal Antibody - 5 Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TBR1 antibody (AWA10109) at 1/200 dilution.
    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109 at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner
    TBR1 Recombinant Rabbit Monoclonal Antibody - 6 Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TBR1 antibody (AWA10109) at 1/200 dilution.
    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner
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