TBR1 Recombinant Rabbit Monoclonal Antibody

货号：

AWA10109

应用：

WB,IHC-P,IHC-F,IF-T,mIHC,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

74 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

T box brain protein 1; T-box brain protein 1; T box brain 1; T brain 1; T-brain-1; TBR 1, TBR1, TBR-1; Tbr1; TBR1_HUMAN; TES 56; TES-56

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000

IHC-P 1:200-1:1000

IHC-F 1:200-1:1000

IF-T 1:500

mIHC 1:1000

FCM 1:50-1:100

Immunogen

Information

Gene Name：

TBR1

Protein Name：

T-box brain protein 1

Gene ID：

10716 (Human)

21375 (Mouse)

680427 (Rat)

SwissPro：

Q16650 (Human)

Q64336 (Mouse)

D4A6N8 (Rat)

Subcellular Location：

Nucleus.

Immunogen：

Synthetic peptide within Human TBR1. AA range: 30-75.

Specificity:

TBR1 Monoclonal Antibody detects endogenous levels of TBR1 protein.

Product images
	Fig: Western blot analysis of TBR1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA10109, 1/1000) was used in TBST(0.3%TWEEN20) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Predicted molecular weight: 74KD Observed molecular weight: 74KD Exposure time: 90 second
	Fig: Fluorescence immunohistochemical analysis of Rat-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TBR1 antibody (AWA10109) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109 at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner
	Fig: Fluorescence immunohistochemical analysis of Mouse-brain tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-TBR1 antibody (AWA10109) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner
	Fig: Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Rabbit anti-TBR1 (AWA10109) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunohistochemical analysis of paraffin-embedded Rat-brain tissue with Rabbit anti-TBR1 (AWA10109) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10109) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.