α-SMA Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA02626

应用：

WB,IHC,IF,FCM,ELISA

反应性：

Human,Mouse,Rat,Monkey

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat,Monkey

Molecular Wt:

42 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Other Names:

ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC 1:200-1:1000

IF 1:200-1:1000

FCM 1:200-1:400

ELISA 1:10000

Immunogen

Information

Gene Name：

ACTA2

Protein Name：

Actin, aortic smooth muscle

Gene ID：

59 (Human)

11475 (Mouse)

81633 (Rat)

SwissPro：

P62736 (Human)

P62737 (Mouse)

P62738 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

Synthesized peptide of human α-SMA.

Specificity:

α-SMA Monoclonal Antibody detects endogenous levels of α-SMA protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-Kidney tissue with Mouse anti-α-SMA antibody (AWA02626) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA02626) at 1/800 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of α-SMA on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA02626, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue Lane 2: Mouse kidney tissue Lane 3: Mouse testis tissue Lane 4: Rat testis tissue Lane 5: HepG2 cell Lane 6: Rat lung tissue Lane 7: SK-N-SH cell Lane 8: Mouse spleen tissue Lane 9: Rat spleen tissue Predicted molecular weight:42 kDa Observed molecular weight:42 kDa Exposure time：3 min
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-jejunum tissue with Mouse anti-αSMA antibody (AWA02626) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA02626) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.