Bcl-2 Mouse Monoclonal Antibody

货号:
AWA00392
应用:
WB,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    26 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG2b

    Concentration:

    1mg/ml


    Other Names:

    BCL2; Apoptosis regulator Bcl-2; Bcl-2


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:1000-1:2000
    IF 1:50-1:200
    ELISA 1:1000-1:5000



    Immunogen

    Information

    Gene Name:

    BCL2

    Protein Name:

    Apoptosis regulator Bcl-2


    Gene ID:

    596 (Human)    
    12043 (Mouse)    
    24224 (Rat)

    SwissPro:

    P10415 (Human)    
    P10417 (Mouse)    
    P49950 (Rat)


    Subcellular Location:

    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. Cytoplasm.


    Immunogen:

    Synthetic Peptide of human Bcl-2. AA range: 1-100.


    Specificity:

    Bcl-2 Monoclonal Antibody detects endogenous levels of Bcl-2 protein.




    Product images
    Bcl-2 Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA00392, 1/3000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SHZ-88 cell
    Lane 2: 4T1 cell

    Predicted molecular weight: 26 kDa
    Observed molecular weight: 26 kDa
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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