PDXK Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA00236

应用：

WB,IHC-P,IF,FCM

反应性：

Human,Mouse

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse

Molecular Wt:

35 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

1 mg/ml

Other Names:

C21orf124; C21orf97; PDXK; PKH; PNK; PRED79; Pyridoxal kinase; Pyridoxine kinase; HMSN6C; HEL-S-1a

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:200-1:1000

IF 1:50-1:200

FCM 1:200-1:400

Immunogen

Information

Gene Name：

PDXK

Protein Name：

Pyridoxal kinase

Gene ID：

8566 (Human)

216134 (Mouse)

SwissPro：

O00764 (Human)

Q8K183 (Mouse)

Subcellular Location：

Cytoplasm, cytosol.

Immunogen：

Recombinant fragment of human PDXK. AA range: 1-312.

Specificity:

PDXK Monoclonal Antibody detects endogenous levels of PDXK protein.

Product images
	Fig : Western blot analysis of PDXK on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00236, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis Lane 2: Mouse heart Lane 3: RT-4 cell Lane 4: 786-O cell Lane 5: Mouse kidney Lane 6: A549 cell Lane 7: LLC cell Lane 8: SK-OV-3 cell Lane 9: DU145 cell Predicted molecular weight:35 kDa Observed molecular weight:35 kDa Exposure time：3 min
	Fig: Fluorescence immunohistochemical analysis of Rat-spinal cord tissue (Formalin/PFA-fixed paraffin-embedded sections) with mouse anti-PDXK antibody (AWA00236) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00236) at 1/200 dilution for 2 hour at 37℃ or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (Red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.