P53 Mouse Monoclonal Antibody

货号:
AWA00214
应用:
WB
反应性:
Human
来源:
Mouse
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Mouse

    Reactivity:

    Human,Rat

    Molecular Wt:

    Predicted MW: 44 kDa

    Observed MW: 53 kDa

     


    Clonality:

    Monoclonal

    Isotype:

    IgG2a



    Other Names:

    TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; BCC7; LFS1; BMFS5; TRP53

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000



    Immunogen

    Information

    Gene Name:

    TP53

    Protein Name:

    Cellular tumor antigen p53


    Gene ID:

    7157 (Human)        

    24842 (Rat)

    SwissPro

    P04637 (Human)        

    P10361 (Rat)

     


    Subcellular Location:

    Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

     


    Immunogen:

    Synthesized peptide derived from human P53. AA range: 250-393.


    Specificity:

    P53 Monoclonal Antibody detects endogenous levels of P53 protein.


    Product images
    P53 Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of P53 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00214, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: PC-12 cell
    Lane 2:A2058 cell
    Lane 3: A375 cell
    Lane 4: RBL-2H3 cell
    Lane 5: HCT116 cell
    Lane 6: HEK-293 cell

    Calculated molecular weight: 44kDa
    Observed molecular weight: 53kDa
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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