TBK1 Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA00198

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

84 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

2 mg/ml

Other Names:

TBK1; NAK; Serine/threonine-protein kinase TBK1; NF kappa B activating kinase; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; TANK binding kinase 1; FLJ11330

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:1000

IHC-P 1:200

Immunogen

Information

Gene Name：

TBK1

Protein Name：

Serine/threonine-protein kinase TBK1

Gene ID：

29110 (Human)

56480 (Mouse)

299827 (Rat)

SwissPro：

Q9UHD2 (Human)

Q9WUN2 (Mouse)

D4A7D3 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

Recombinant protein within human TBK1. AA range: 530-729.

Specificity:

TBK1 Monoclonal Antibody detects endogenous levels of TBK1 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Mouse anti-TBK1 (AWA00198) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00198) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-stomach tissue with Mouse anti-TBK1 (AWA00198) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00198) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of TBK1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00198, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti--Mouse IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: Raw264.7 cell Lane 3:K562 cell Lane 4:NIH3T3 cell Lane 5:Rat brain tissue Lane 6:Mouse brain tissue Lane 7:4T1 cell Lane 8:U2OS cell Lane 9:A549 cell Lane 10:HEK-293 cell Lane 11Rat stomach tissue Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time:3min