DRP1 Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA00145

应用：

WB,IHC-P,IF,FCM,ELISA

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

82 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Other Names:

DNM1L; DLP1; DRP1; Dynamin-1-like protein; Dnm1p/Vps1p-like protein; DVLP; Dynamin family member proline-rich carboxyl-terminal domain less; Dymple; Dynamin-like protein; Dynamin-like protein 4; Dynamin-like protein IV; HdynIV; Dynamin-rela

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:100-1:500

IF 1:200-1:1000

FCM 1:200-1:400

ELISA 1:10000

Immunogen

Information

Gene Name：

DNM1L

Protein Name：

Dynamin-1-like protein

Gene ID：

10059 (Human)

74006 (Mouse)

114114 (Rat)

SwissPro：

O00429 (Human)

Q8K1M6 (Mouse)

O35303 (Rat)

Subcellular Location：

Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

Immunogen：

Recombinant fragment of human DRP1. AA range: 69-213.

Specificity:

DRP1 Monoclonal Antibody detects endogenous levels of DRP1 protein.

Product images
	Fig: Immunocytochemistry analysis of NIH3T3 cells labeling DRP1 with Mouse anti-DRP1 antibody (AWA00145) at 1/50 dilution(Red ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-DRP1 antibody (AWA00145) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of DRP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00145, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: LLC cell Lane 2: RAW264.7 cell Lane 3: PC-12 cell Lane 4: Rat brain tissue Predicted molecular weight:82KD Observed molecular weight:75-85KD Exposure time:90 sec
	Fig : Western blot analysis of DRP1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00145, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell Lane 2: HEK-293 cell Lane 3: HCT116 cell Lane 4: Hela cell Lane 5: HepG2 cell Lane 6: SH-SY5Y cell Lane 7: A431 cell Lane 8: K562 cell Lane 9: NIH3T3 cell Lane 10: Mouse brain tissue Predicted molecular weight:82KD Observed molecular weight:75-85KD Exposure time:90 sec
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue with Mouse anti-DRP1 (AWA00145) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00145) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-forehead tissue with Mouse anti-DRP1 (AWA00145) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00145) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Mouse anti-DRP1 (AWA00145) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00145) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.