IL-1β Mouse Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA00115

应用：

WB,ELISA

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

17,28,31 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Other Names:

Interleukin-1 beta (IL-1 beta) (Catabolin); IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

ELISA 1:5000-1:20000

Immunogen

Information

Gene Name：

IL1B

Protein Name：

Interleukin-1 beta

Gene ID：

3553 (Human)

16176 (Mouse)

24494 (Rat)

SwissPro：

P01584 (Human)

P10749 (Mouse)

Q63264 (Rat)

Subcellular Location：

Extracellular exosome, Secreted, Lysosome, Cytosol.

Immunogen：

Synthesized peptide derived from human IL-1β. AA range: 150-250.

Specificity:

IL-1β Monoclonal Antibody detects endogenous levels of IL-1β protein.

Product images
	Fig : Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA00115, 1/5000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate
	Fig : Western blot analysis of IL-1β on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00115, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell Lane 2: THP-1 cell(THP-1 treated with 80 nM TPA overnight and then 100 ng/ml LPS for 6 h and 300 ng/ml Brefeldin A for the last 3 h) Lane 3:Raw264.7 Lane 4:Raw264.7(treated with 100 ng/ml LPS for 7 hours and 300 ng/ml Brefeldin A for the last 3 hours)