MAP2 Mouse Monoclonal Antibody

货号:
AWA00067
应用:
WB,IHC-P,IF-C
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    200 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1mg/ml


    Other Names:

    MAP 2; MAP-2; MAP2; Microtubule-associated protein 2; Microtubule associated protein 2; MAP2A; MAP2B; MAP2C; DKFZp686I2148; MAP dendrite specific


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-2000
    IHC-P 1:100-1:500
    IF-C 1:50-200



    Immunogen

    Information

    Gene Name:

    MAP2

    Protein Name:

    Microtubule-associated protein 2


    Gene ID:

    4133 (Human)    
    17756 (Mouse)    
    25595 (Rat)

    SwissPro:

    P11137 (Human)    
    P20357 (Mouse)    
    P15146 (Rat)


    Subcellular Location:

    Cytoplasm, cytoskeleton. Cell projection, dendrite.


    Immunogen:

    Synthetic Peptide of MAP2.


    Specificity:

    MAP2 Monoclonal Antibody detects endogenous levels of MAP2 protein.




    Product images
    MAP2 Mouse Monoclonal Antibody - 1 Fig: Immunocytochemistry analysis of SH-SY5Y cells labelingMAP2 with Mouse anti-MAP2 antibody (AWA00067)at 1/50 dilution(Red).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-MAP2 antibody (AWA00067)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 567, AWS0004c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    MAP2 Mouse Monoclonal Antibody - 2 Fig : Western blot analysis of MAP2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00067, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell

    Predicted molecular weight:200KD
    Observed molecular weight:200KD

    Exposure time:45 sec
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
    细胞描述: GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆,而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素,也可产生
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